反转录突变体的2号内含子部分和3号外显子开头部分被替换了反向排列的诺卡霉素抗性基因。通过实时PCR和 Western blot 实验,证明了纯合子中无基因产物存在。(来源:J:86750)
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Part of intron 2 and the first part of exon 3 were replaced by a neomycin resistance gene in the reverse orientation. Lack of gene product in homozygotes was demonstrated by both real-time PCR and Western blot analysis. (J:86750)