Tg(PLFSADA)2465Rkmb

A construct was made that included a 2.8 kb genomic fragment containing the endogenous polyadenylation sequence, intron 11, and ~2kb of 3'-untranslated region and 3' flanking region of the gene. The endogenous promoter was replaced with a promoter containing a 36-bp deletion in the 5'-untranslated region. This resulted in a construct that contained the gene's regulatory elements, allowing expression in the placenta prenatally and the forestomach postnatally, while the 36-bp deletion in the promoter allowed distinction of this transcript from that of native transcript. RNase protection showed transgene transcript in placentas, but not fetuses, of transgenic animals. Enzymatic activity of the protein product was increased in the placenta, but not in the fetus, of transgenic mice compared to nontransgenic animals. (J:29350)