将包含BH3交互域的exons 2和3替换为一个带有floxed PGK-neomycin表达元件的构建。在体内通过与C57BL/6的Deleter小鼠品系杂交移除抗性基因。基因打靶通过RT-PCR和Northern blot实验进行了确认。(来源:J:86466)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count