将包含两个BH3结合域的exons 2和3替换为一个带有floxed PGK-hygromycin抗性标记的载体。抗性基因通过与C57BL/6的Deleter小鼠杂交在体内去除。基因打靶通过RT-PCR和Northern blot分析进行了确认。(来源:J:86466)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count