Krt71^Ca-Rin

Sequence analysis identified the spontaneous deletion of asparagine codon 139 or 140, through the deletion of CAA / ACA / AAC from the AACAACAA sequence at codong nucleotides 415-422. The deleted codon is located in the alpha-helical rod domain. Reduced levels of transcript were detected by Northern blot and RT-PCR analyses of RNA isolated from the skin of 5 week old homozygous mutant mice. Immunohistochemical analysis showed protein in the inner root sheath and, ectopically, in the hair shaft, indicating abnormal protein localization. (J:86407)