在体外通过Cre介导的剪切后,Exon 11及其周边的内含子序列被替换为一个LoxP位点。在纯合突变小鼠中,通过RNA酶保护和 Western blot 分析,未检测到正常转录本或蛋白质。(来源:J:86218)
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Exon 11 and flanking intronic sequence was replaced with a single loxP site after cre-mediated excision in vitro. Neither normal transcript nor protein were detected in homozygous mutant mice by RNase protection and Western blot analysis. (J:86218)