包含起始密码子的外显子通过同源重组被抗氨苄青霉素选择标记 cassette 替换。 Western blot 检测结果显示,纯合小鼠脑组织中未检测到可检测的蛋白质。(来源:J:57368)
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The exon containing the translation initiation codon was replaced with a neomycin selection cassette by homologous recombination. Western blot analysis confirmed that no detectable protein was present in brain extracts of homozygous mice. (J:57368)