从1号外显子到3号外显子被替换了,通过同源重组插入了诺姆西林选择标记。在 Western blot 分析中,纯合突变小鼠中未检测到该蛋白质。(来源:J:86213)
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Exons 1 through 3 were replaced with a neomycin selection cassette inserted by homologous recombination. Protein was undetected in homozygous mutant mice by Western blot analysis. (J:86213)