启动子区域和编码区域的5'端被替换成一个反向排列的诺卡因抗性基因。通过RT-PCR检测未发现mRNA转录,通过 Western blot确认无蛋白产物存在。(来源:J:85937)
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The promotor region and the 5' end of the coding region were replaced by a neomycin resistance gene in inverse orientation. Absence of mRNA transcripts was demonstrated by RT-PCR and absence of protein product by Western blot. (J:85937)