Fos^tm7Wag

A loxP site was inserted in the first intron and a PGK neomycin cassette flanked by flp sites was inserted along with a second loxP site downstream of the polyadenylation signal. Still further downstream, a splice acceptor was placed just 5' to an EGFP gene insert. The PGK neo construct was deleted in vivo using flip recombinase transgenic mice. (J:85928)