所有exon 1和exon 2的5'端部分被通过同源重组插入了诺姆西林选择标记 cassette,用 Western blot分析同源纯合突变ES细胞时未检测到蛋白质。(来源:J:85708)
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All of exon 1 and a 5' portion of exon 2 was replaced with a neomycin selection cassette inserted by homologous recombination. Protein was undetected by Western blot analysis of homozygous mutant ES cells. (J:85708)