内源性位点由插入的包含GFP和neoR的 cassette打断。通过对来自纯合突变小鼠的巨噬细胞进行 Western blot 分析,未检测到蛋白质。(来源:J:85746)
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The endogenous locus was disrupted by the insertion of a cassette containing GFP and neoR. Protein was undetected by Western blot analysis of macrophages obtained from homozygous mutant mice. (J:85746)