从起始密码子开始不久,exon 1被反向插入了抗氨苄青霉素抗性基因 cassette。对肝脏蛋白提取物的 Western blot 检测确认了没有翻译产物产生。(来源:J:85442)
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Starting shortly after the start codon, exon 1 was replaced by a neomycin resistance cassette in opposite orientation. Western blot analysis of liver protein extracts confirmed the absence of translation product. (J:85442)