在替换了包括第三外显子的基因组序列后,使用了新霉素筛选 cassette。对纯合小鼠视网膜匀浆进行的 Western blot 分析显示,这个等位基因不产生可检测到的蛋白质。(来源:J:33048)
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A neomycin selection cassette replaced genomic sequences including the third exon. Western blot analysis on retinal homogenates derived from homozygous mice demonstrated that no detectable protein was produced from this allele. (J:33048)