一种新霉素筛选 cassette 替换了包括3到5号染色体的所有外显子以及6号部分外显子的序列。通过 Western blot 分析脾脏细胞总蛋白提取,发现纯合突变小鼠中未检测到该蛋白质(来源:J:77049, J:144438)。
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A neomycin selection cassette replaced sequence encompassing all of exons 3 through 5 and a portion of exon 6. Protein was undetected in homozygous mutant mice by Western blot analysis of spleen whole-cell protein extracts. (J:77049, J:144438)