内源性位点通过同源重组方式插入了PGK-neo cassette后发生了中断。删除区域包含起始密码子。在纯合突变小鼠的 Western blot 分析中未检测到蛋白质。(来源:J:71179)
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The endogenous locus was disrupted by a PGK-neo cassette inserted by homologous recombination. The deleted region included the start codon. Protein was undetected by Western blot analysis of homozygous mutant mice. (J:71179)