通过同源重组插入的新内切录基因替换了编码催化活性必需的亚域XI的exon。对纯合突变小鼠脾细胞进行 Western blot 分析后,未检测到该蛋白质。(来源:J:85266)
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A neo cassette inserted by homologous recombination replaced the exon encoding subdomain XI which is essential for catalytic activity. Protein was undetected by Western blot analaysis of splenocytes obtained from homozygous mutant mice. (J:85266)