通过同源重组,Neomycin选择基因替换了C和D外显子, Western blot结果显示,来自纯合小鼠细胞的胞质中未检测到可检测到的蛋白质产品。(来源:J:57946)
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A neomycin selection gene replaced exons C and D by homologous recombination. Western blot analysis demonstrated that no detectable protein product was made in the cytosol of cells derived from homozygous mice. (J:57946)