Tg(Thy1-MAPT)183Gotz

Sequence encoding the longest human MAPT brain isoform (tau40), consisting of exons 2 and 3 as well as four microtubule-binding repeats, was adjoined to the murine Thy1 promoter. The human MAPT sequence included the P301L point mutation, which is linked to human frontotemporal dementia with parkinsonism. Soluble levels of transgenic protein in the brain were approximately 70% of the endogenous level. Expression levels of transgenic protein were high in the hippocampus, fornix fimbriae, amygdala, spinal cord, and cortex, weaker in brain stem and striatum, and not detectable in the olfactory bulb and cerebellum. (J:66831)