Exon 1被替换为包含IRE-S序列和lacZ neo的 cassette,通过在纯合突变小鼠的骨髓中进行RT-PCR和 Western blot分析,未检测到转录和蛋白质表达(来源:J:84680)
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Exon 1 was replaced with a cassette containing IRES-lacZ and neo. Transcript and protein were undetected in homozygous mutant mice by RT-PCR and Western blot analyses of bone marrow. (J:84680)