将含有GFP和IRES-neo的靶向载体通过同源重组后,成功替换了从外显子4末端到部分外显子5的基因组片段。目标位点被设计成编码一个由内源基因后面连接8个氨基酸和GFP组成的381氨基酸蛋白。通过在纯合突变小鼠中进行 Western blot 分析,检测到了预期大小的蛋白质存在。(来源:J:84634)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S1/Sv-Oca2+ Tyr+ Kitl+
Targeted
插入,基因内删除
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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