从1号外显子到7号外显子的序列被替换,编码了Cre重组酶和neo,但通过在纯合突变胚胎中进行 Western blot 分析,未检测到该蛋白质。相反,Cre重组酶通过内源性启动子表达。(来源:J:87043)
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Exons 1 through 7 were replaced with sequence encoding cre recombinase and neo. Protein was undetected by Western blot analysis of homozygous mutant embryos. Instead, Cre recombinase is expressed via the endogenous promoter. (J:87043)