一段从1到5号外显子的序列被通过同源重组插入了诺姆林选择标记。对红细胞的 Western blot 检测显示,纯合突变小鼠中没有蛋白质表达。(来源:J:84271)
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Sequence encompassing exons 1 through 5 was replaced with a neomycin selection cassette inserted by homologous recombination. Western blot analysis of RBCs indicated an absence of protein in homozygous mutant mice. (J:84271)