在exon 1的开放阅读框(氨基酸1-46)被替换为抗羟氨苄青霉素-绿色荧光蛋白融合基因。对E9.5同源胚胎的 Western blot 检测结果显示无基因产物存在。(来源:J:84329)
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The open reading frame in exon 1 (amino acids 1-46) was replaced with a hygromycin resistance-EGFP fusion gene. Western blot analysis of E9.5 homozygous embryos proved the absence of gene product. (J:84329)