1和2号外显子被替换成抗氨苄青霉素药耐药基因和lacZ基因。RT-PCR结果显示纯合子肝脏中无mRNA,而 Western blot 和免疫组化也证实了无蛋白质存在。(来源:J:84376)
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Exons 1 and 2 were replaced with a neomycin drug resistance gene and a lacZ gene. RT-PCR demonstrated the absence of mRNA in the livers of homozygotes while the absence of protein was proven by Western blot and by immunohistochemistry. (J:84376)