Exon 2,包含PH域的部分,被替换为抗氨苄青霉素抗性基因。通过PCR和 Western blot在杂合子小鼠和纯合胚胎中确认了同源重组。(来源:J:83953)
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Exon 2, which contains part of the PH domain, was replaced with a neomycin resistance gene. Homologous recombination was confirmed in heterozygous mice and homozygous embryos by PCR and by Western blots. (J:83953)