Tg(PAH-INSR)1Rjv

The transgene consists of a human PAH promoter and a sequence endcoding a truncated and activated from of human INSR. The mutant INSR contains the first 12 amino acids of the alpha subunit fused to 20% of the C-terminal portion of the alpha subunit and the entire beta subunit. Strong transgene expression in the liver (approximately 6.5-fold higher than endogenous mouse mRNA) was confirmed by RT-PCR and Western blot. Weak transgene expression was also observed in kidneys and testis. (J:80773)