将含有诺他霉素抗性基因和胸苷激酶基因,这两者被loxP位点包裹的盒式元件插入了第2内含子。在第3内含子中也放置了一个loxP位点。Southern blot实验确认了同源重组。将neoTK盒式元件插入Men1基因(T等位基因)的第2内含子,通过RT-PCR和 Western blot分别在RNA和蛋白水平上干扰了Men1的表达(来源:J:83882)。

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Targeted
插入
--
1
23
11

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
Show/Hide columns
Phenotypes

References Literature

Title
PMID
Journal
Year
IF
No Data Found!
微信
信息比对
科研助手
使用教程
回到顶部