外显子1被删除,并通过同源重组替换为lacZ-PGK-neo的内切接子。基因表达缺失通过分析野生型动物大脑蛋白的 Western blot 来确认。(来源:J:83752)
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Exon 1 was deleted and replaced with an in-frame lacZ-PGK-neo cassette via homologous recombination. Absence of gene expression was confirmed by Western blot analysis of brain proteins from homozygous mutant animals. (J:83752)