这个基因通过同源重组在exon 1位置插入了PGK-neo cassette,导致其功能受损。通过在纯合突变动物胸腺蛋白中的 Western blot 分析,确实证实了基因表达的缺失。(来源:J:83651)
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The gene was disrupted by insertion of a PGK-neo cassette into exon 1 via homologous recombination. Absence of gene expression was confirmed by Western blot analysis of thymus proteins from homozygous mutant animals. (J:83651)