整个编码区域被通过同源重组插入的新 Cas9 镜像元件替换。对脂肪、肝脏和肌肉组织的 Western 印迹分析显示,纯合突变小鼠中该蛋白质水平降低。(来源:J:83297)
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The entire coding region was replaced with a neo cassette inserted by homologous recombination. Western blot analysis of adipose, liver, and muscle tissue showed reduced levels of protein in homozygous mutant mice. (J:83297)