这个基因通过同源重组的方式,插入了一个内切位点lacZ-neo cassette,导致其功能受损。通过 Western blot 对皮肤蛋白的分析,确实证实了纯合突变动物中基因表达的缺失。(来源:J:83058)
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The gene was disrupted by insertion of an in-frame lacZ-neo cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by Western blot analysis of skin proteins. (J:83058)