1号外显子的5'端被删除,并通过同源重组替换为诺卡因抗性基因座。通过 Western blot 分析,证实了纯合突变动物中基因表达的缺失。(来源:J:64745)
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The 5' portion of exon 1 was deleted and replaced with a neomycin resistance cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by Western blot analysis. (J:64745)