5'端的第六外显子被通过同源重组插入了一个neo cassette,用 Western blot 分析同源纯合突变 ES 细胞的提取物时未检测到该蛋白质。(来源:J:46239)
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A 5' portion of exon 6 was replaced with a neo cassette inserted by homologous recombination. Protein was undetected by Western blot analysis of extracts from homozygous mutant ES cells. (J:46239)