Dnmt1^tm3Jae

A targeting vector containing a Dnmt1 cDNA fragment followed by a PGK-puro cassette was inserted upstream of the previously targeted coding region containing a neomycin selection cassette (Dnmt1tm1Enl) and downstream of the endogenous promoter. Northern blot analysis showed that transcript produced by the inserted cDNA sequence was at a level and size similar to that of the endogenous allele. (J:83179)