部分exon1和2,以及全部内含子1被替换成含有lacZ和neo的 cassette。通过免疫荧光和 Western blot分析,证实了纯合突变小鼠中正常蛋白的缺失。(来源:J:59165)
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Portions of exons 1 and 2 and all of intron 1 were replaced with a cassette containing lacZ and neo. The absence of normal protein in homozygous mutant mice was verified by immunofluorescence and Western blot analysis. (J:59165)