这个基因通过同源重组的方式,用诺卡因抗性基因 cassette 替换了前3个外显子,导致其失活。通过 Western blot 分析,我们确认了纯合突变动物中基因表达的缺失。(来源:J:82516)
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The gene was disrupted by replacement of the first 3 exons with a neomycin resistance cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by Western blot analysis. (J:82516)