通过同源重组靶向载体,删除了起始密码子和信号肽的序列。来自纯合突变小鼠血清的 Western blot 检测结果显示没有蛋白质表达。(来源:J:82376)
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Sequence encoding the start codon and signal peptide was deleted by homologous recombination of a targeting vector. Western blot analysis of sera obtained from homozygous mutant mice indicated an absence of protein. (J:82376)