App^tm1.1Cep

2 missense mutations (K670N and M671L), originally identified in a Swedish kindred susceptible to familial Alzheimer's disease (FAD), were introduced into exon 16 via homologous recombination. Mutant protein was identified by Western blot analysis of mutant brain extracts. An intronic floxed neo cassette that was included in the targeting vector for selection was excised prior to the generation of chimeric mice. (J:35500)