内源性位点通过同源重组插入了lacZ-neo cassette后发生了失活。在纯合突变脾脏的 Western blot 分析中未检测到正常蛋白。(来源:J:63794)
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The endogenous locus was disrupted by a lacZ-neo cassette inserted by homologous recombination. Normal protein was undetected by Western blot analysis of homozygous mutant spleens. (J:63794)