Exon 2被替换为通过同源重组插入的floxed neo cassette,这些异源突变ES细胞提取物的 Western blot分析未检测到正常蛋白。(来源:J:72053)
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Exon 2 was replaced with a floxed neo cassette inserted by homologous recombination. Normal protein was undetected by Western blot analysis of extracts obtained from homozygous mutant ES cells. (J:72053)