内源性等位基因在插入包含9到13号外显子、选择基因以及11号内含子11处300bp缺失的载体后被干扰。通过载体的同源重组,产生了双链断裂修复的等位基因,特征是13号外显子后面跟着选择基因,9到13号外显子有重复。通过睾丸和脾脏组织的RT-PCR分析,杂合突变小鼠中未检测到转录本。邻近基因Vrk2的表达未受影响。(来源:J:80424)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S7/SvEvBrd-Hprt1+
Targeted
插入,基因内删除
--
1
5
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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