内源性等位基因在起始密码子下游的β- gal 和 neo 22 框插入突变,导致蛋白质缺失,通过免疫荧光法在纯合突变小鼠中得到了体现(来源:J:81797)
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The endogenous allele was disrupted by the in frame insertion of Beta-gal and neo 22 codons downstream of the start codon. An absence of protein in homozygous mutant mice was indicated by immunofluorescence. (J:81797)