通过将突变动物与Cre敲除品系杂交,随后去除了第1外显子(Floxed exon 1)。通过 Western blot 对大脑蛋白进行分析,确实证实了纯合突变动物中基因表达的缺失(来源:J:81845)。
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Floxed exon 1 was subsequently removed by crossing mutant animals to a cre deleter strain. Absence of gene expression in homozygous mutant animals was confirmed by Western blot analysis of brain proteins. (J:81845)