这个基因通过同源重组插入了耐药性诺卡因 cassette,导致其功能受损。通过 Western blot 分析,我们确认了纯合子突变肾中没有基因表达(来源:J:81814)。
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The gene was disrupted by insertion of a neomycin resistance cassette via homologous recombination. Absence of gene expression homozygous mutant kidneys was confirmed by Western blot analysis. (J:81814)