Hspg2^tm1Soin

Exon 3 and flanking regions were deleted and replaced with a PGK-neo cassette via homologous recombination. The targeted mutation removes the heparan sulfate attachment while the core protein remains intact. RT-PC analysis of RNA from homozygous mutant embryos confirmed the exon 3 deletion. Fibroblasts derived from homozygous mutant cell culture were treated with heparitinase and chondroitinase, run on an SDS-PAGE gel, and immunoblotted to confirm the mutant protein retained the core protein and lacked heparan sulfate side chains. (J:81399)