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The gene was disrupted by replacement of exon 1 and part of intron 1 with a PGK-HPRT cassette via homologous recombination. Absence of normal transcripts was confirmed by Northern blot analysis of ovary mRNA from homozygous mutant animals using the full cDNA as a probe. Longer exposure of the Northern blot revealed a smaller transcript of unknown relevance. (J:81447)