Ntan1^tm1Avar

The gene was disrupted by replacement of exons 2-5 with a PGK-neo cassette via homologous recombination. Northern blot analysis detected no expression in brains of homozygous mutant animals, but testes mRNA contained transcripts which apparently lack the 5' half of the open reading frame. Whole mount in-situ hybridization analysis of E9.75 homozygous mutant embryos did not detect any gene expression. (J:62140)