通过同源重组,将增强型绿色荧光蛋白(GFP)序列插入了内源性位点的第35位密码子。除了插入,还去除了包含同源盒域前段(141-184个氨基酸)的序列,从而引入了frameshift突变。GFP的表达与内源基因相似。(来源:J:81343)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S1/Sv-Oca2+ Tyr+ Kitl+
Targeted
插入,基因内删除
--
1
23
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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