将连接了Cre重组酶的内含子插入到原基因3' UTR中,通过同源重组。经Frt标记的Hygromycin筛选 cassette切除后,UTR中保留了一个Frt座。未观察到等位基因补救现象,表明插入序列对原基因表达影响不大。(来源:J:80725)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S1/Sv-Oca2+ Tyr+ Kitl+
Targeted
插入
--
1
23
72

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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